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rabbit polyclonal antiserum against synthetic rat p2x3  (Neuromics)


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    Neuromics rabbit polyclonal antiserum against synthetic rat p2x3
    Rabbit Polyclonal Antiserum Against Synthetic Rat P2x3, supplied by Neuromics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p2x3+polyclonal+rabbit+antiserum/rabbit+anti+p2x3/pm20378247-119-60-68
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antiserum against synthetic rat p2x3 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Isolation:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Blocking Assay:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Real-time Polymerase Chain Reaction:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Amplification:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Immunofluorescence:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Labeling:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Recombinant:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Injection:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Concentration Assay:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Produced:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Activation Assay:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.
    Article Snippet: Confocal images were restored to grayscale and optimized for contrast.Confocal images were restored to grayscale and optimized for contrast.. P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).. Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.Antibody specificity was confirmed by omission of the primary antibody and by pre-adsorption of the primary antibody with the target peptide.



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    Fig. 3. Analysis of P2X receptor expression in neonatal rat SGN. (A) Isolation of a single SGN from a P4 rat cochlear slice using a micropipette. (B) Example of an inward current response to the P2X3 and P2X2/3 receptor agonist ,MeATP (100 M) in a SGN using whole-cell voltage clamp (holding potential –60 mV). (C) Block of the ATP response (100 M, 5 seconds of focal application) by the P2X2/3 receptor-specific antagonist A- 317491 (500 nM, bath superfusion). (D) Real-time PCR amplification plot showing detection of P2X3 cDNA in a sample of individual SGN. (E) Average transcript copy number for each P2X receptor subunit and the housekeeping genes Nse and Gapdh in individual neurons. Note that the P2X3 transcript number was twice that of P2X2 (*P<0.01; P2X3 transcript number was significantly greater than the other P2X subunits; GAPDH transcript copy number was significantly greater than NSE transcript copy number). (F) Relative distribution of P2X receptor subunits in the population of SGN. This plot shows the normalized mRNA transcript copy number for each of the seven candidate P2X receptor subunits expressed by individual SGN. (G) Immunofluorescence labeling of P2X2, P2X3 and P2X4 in neonatal (P4) spiral ganglion tissue.

    Journal: Development (Cambridge, England)

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.

    doi: 10.1242/dev.002279

    Figure Lengend Snippet: Fig. 3. Analysis of P2X receptor expression in neonatal rat SGN. (A) Isolation of a single SGN from a P4 rat cochlear slice using a micropipette. (B) Example of an inward current response to the P2X3 and P2X2/3 receptor agonist ,MeATP (100 M) in a SGN using whole-cell voltage clamp (holding potential –60 mV). (C) Block of the ATP response (100 M, 5 seconds of focal application) by the P2X2/3 receptor-specific antagonist A- 317491 (500 nM, bath superfusion). (D) Real-time PCR amplification plot showing detection of P2X3 cDNA in a sample of individual SGN. (E) Average transcript copy number for each P2X receptor subunit and the housekeeping genes Nse and Gapdh in individual neurons. Note that the P2X3 transcript number was twice that of P2X2 (*P<0.01; P2X3 transcript number was significantly greater than the other P2X subunits; GAPDH transcript copy number was significantly greater than NSE transcript copy number). (F) Relative distribution of P2X receptor subunits in the population of SGN. This plot shows the normalized mRNA transcript copy number for each of the seven candidate P2X receptor subunits expressed by individual SGN. (G) Immunofluorescence labeling of P2X2, P2X3 and P2X4 in neonatal (P4) spiral ganglion tissue.

    Article Snippet: P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).

    Techniques: Expressing, Isolation, Blocking Assay, Real-time Polymerase Chain Reaction, Amplification, Immunofluorescence, Labeling

    Fig. 5. Pharmacology of the recombinant P2X2-3/3 receptor. (A) Injection of P2X2-3 and P2X3 mRNAs in a ratio of 1:2 into Xenopus oocytes resulted in expression of ATP-gated inward currents with a broad sensitivity to ATP agonists. (B) Concentration-response curves for ATP at pH 7.5 show an EC50 of 0.4 M for these ATP-gated ion channels. Acidification to pH 6.5 produced a leftward shift in the concentration-response curve, to reduce activation thresholds to <10 nM ATP. This is attributable to positive allosteric modulation by protons acting through the P2X2 subunit.

    Journal: Development (Cambridge, England)

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.

    doi: 10.1242/dev.002279

    Figure Lengend Snippet: Fig. 5. Pharmacology of the recombinant P2X2-3/3 receptor. (A) Injection of P2X2-3 and P2X3 mRNAs in a ratio of 1:2 into Xenopus oocytes resulted in expression of ATP-gated inward currents with a broad sensitivity to ATP agonists. (B) Concentration-response curves for ATP at pH 7.5 show an EC50 of 0.4 M for these ATP-gated ion channels. Acidification to pH 6.5 produced a leftward shift in the concentration-response curve, to reduce activation thresholds to <10 nM ATP. This is attributable to positive allosteric modulation by protons acting through the P2X2 subunit.

    Article Snippet: P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).

    Techniques: Recombinant, Injection, Expressing, Concentration Assay, Produced, Activation Assay

    Fig. 6. Developmental profile of P2X receptor subunit expression in rat spiral ganglion tissue from P0-P14, determined by end- point RT-PCR. Note the downregulation of the P2X3 transcript by P14.

    Journal: Development (Cambridge, England)

    Article Title: P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea.

    doi: 10.1242/dev.002279

    Figure Lengend Snippet: Fig. 6. Developmental profile of P2X receptor subunit expression in rat spiral ganglion tissue from P0-P14, determined by end- point RT-PCR. Note the downregulation of the P2X3 transcript by P14.

    Article Snippet: P2X3 polyclonal rabbit antiserum (Neuromics, Bloomington, MN) was targeted to residues 383-397 (VEKQSTDSGAYSIGH), P2X4 polyclonal rabbit antiserum (Alomone Laboratories, Israel) was targeted to residues 370-388 (YVEDYEQGLSGEMNQ), and P2X2 was targeted to residues 96-113 (VSIITRIEVTPSQTLGTC) (Kanjhan et al., 1996).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction